ATTO550, ATT0647N specs

ATTO550: home page, spectra in xls
ATTO647N: home page, spectra in xls



In [1]:

    
atto550_ext_coeff = 1.2*1e5   # 1 / ( mol cm )
atto647N_ext_coeff = 1.5*1e5  # 1 / ( mol cm )



In [2]:

    
%matplotlib inline
import matplotlib.pyplot as plt
import pandas as pd



In [3]:

    
a550 = pd.read_excel('ATTO550.xlsx', 'Tabelle1', index_col=None, na_values=['NA'])
a647N = pd.read_excel('ATTO647N.xlsx', 'Tabelle1', index_col=None, na_values=['NA'])



In [4]:

    
a550[:4]









    Out[4]:






  
    
      
      Absorption
      Unnamed: 1
      Unnamed: 2
      Emission
      Unnamed: 4
    
  
  
    
      0
       Wavelength, nm
       Absorbance
      NaN
       Wavelength, nm
       Intensity, a.u. 
    
    
      1
                  NaN
              NaN
      NaN
                  NaN
                    NaN
    
    
      2
                  250
            0.324
      NaN
                  520
                      0
    
    
      3
                250.2
            0.325
      NaN
                  521
                      0



In [5]:

    
a647N[:4]









    Out[5]:






  
    
      
      Absorption
      Unnamed: 1
      Unnamed: 2
      Emission
      Unnamed: 4
    
  
  
    
      0
       Wavelength, nm
       Absorbance
      NaN
       Wavelength, nm
       Intensity, a.u. 
    
    
      1
                  NaN
              NaN
      NaN
                  NaN
                    NaN
    
    
      2
                  250
            0.082
      NaN
                  600
                  0.003
    
    
      3
                  251
            0.079
      NaN
                  601
                  0.003



In [6]:

    
a550.columns = ['wl_absorption', 'absorption', 'none', 'wl_emission', 'emission']
ab_550 = a550[['wl_absorption', 'absorption']][2:].set_index('wl_absorption').dropna()
em_550 = a550[['wl_emission', 'emission']][2:].set_index('wl_emission').dropna()
atto550 = pd.concat([ab_550, em_550], axis=1)

a647N.columns = ['wl_absorption', 'absorption', 'none', 'wl_emission', 'emission']
ab_647N = a647N[['wl_absorption', 'absorption']][2:].set_index('wl_absorption').dropna()
em_647N = a647N[['wl_emission', 'emission']][2:].set_index('wl_emission').dropna()
atto647N = pd.concat([ab_647N, em_647N], axis=1)



In [7]:

    
atto550.absorption.plot(label='ATTO550 abs')
atto550.emission.dropna().plot(style='--', ax=plt.gca(), label='ATTO550 em')
atto647N.absorption.plot(ax=plt.gca(), label='ATTO647N abs')
atto647N.emission.dropna().plot(style='--', ax=plt.gca(), label='ATTO647N em')
plt.legend()
plt.title('Absorption and emission spectra (normalized)');

To obtain the absorption cross-section we need to normalize by the extinctyion coefficient:



In [8]:

    
atto550.absorption *= atto550_ext_coeff
atto647N.absorption *= atto647N_ext_coeff



In [9]:

    
atto550.absorption.plot(label='ATTO550 abs', style='b')
atto647N.absorption.plot(ax=plt.gca(), label='ATTO647N abs', style='r')
plt.legend(loc='best')
plt.title('Absorption cross-section');

Absorption cross-section @ 532 nm



In [10]:

    
atto550_abs532 = atto550.absorption.loc[532]
atto647N_abs532 = atto647N.absorption.loc[532]

atto550_abs532, atto647N_abs532









    Out[10]:





(47880.0, 5100.0)



In [11]:

    
ratio = atto647N_abs532 / atto550_abs532
ratio









    Out[11]:





0.10651629072681704



In [12]:

    
with open('../results/Dyes - ATT0647N-ATTO550 abs X-section ratio at 532nm.csv', 'w') as f:
    f.write(str(ratio))

	Absorption	Unnamed: 1	Unnamed: 2	Emission	Unnamed: 4
0	Wavelength, nm	Absorbance	NaN	Wavelength, nm	Intensity, a.u.
1	NaN	NaN	NaN	NaN	NaN
2	250	0.324	NaN	520	0
3	250.2	0.325	NaN	521	0